Paper Information

Journal:   APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY   March 2002 , Volume 69 , Number 2; Page(s) 1 To 16.
 
Paper: 

PURIFICATION AND CHARACTERIZATION OF ANTIFREEZE PROTEIN FROM SUNN PEST (EURYGASTER INTEGRICEPS PUT.).

 
 
Author(s):  BAGHDADI A., MOHARAMIPOUR S., RABANI AZRA, ABD ELAHI GHOLAM ABAS
 
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Abstract: 

Antifreeze protein with thermal hysteresis activity was extracted and purified from adults of Eurygaster integriceps that have been collected trom altitude of Ateshgah Karaj, Iran during winter, and compared with the summer one. Isolation was carried out by using ethanol fractionation, DEAE ion-exchanger chromatography and also more purified with SephadexG-100gel filtration.The puritied protein was also analyzed on SDS polyacrylamide gel electerophoreses for determination of molecular weight. It shows that the molecular weight of this protein is about 19000dalton and its thermal hysterises activity was 4.4°C at concentration of 50 mglml. This protein was absent in summer samples. Therefor, it is suggested that antifreeze protein in winter sample may be an important function in survival of E. integriceps during winter season.

 
Keyword(s): EURYGASTER INTEGRICEPS, ANTIFREEZE PROTEIN, PURIFICATION
 
References: 
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