Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   August 2001 , Volume 37 , Number 1-2; Page(s) 147 To 160.
 
Paper: 

IDENTIFICATION AND PURIFICATION OF A MDMV-LIKE POTYVIRUS OF MAIZE IN MAZANDARAN

 
 
Author(s):  MOINI A. A., IZADPANAH K.A.
 
* 
 
Abstract: 

A potyvirus differing from previously reported maize and sugarcane potyviruses in Iran was isolated from maize in Dasht-e-Naz, Sari, and tentatively designated as "Maize, Mazandaran" (MM). In the host range experiment MM was transmissible to johnsongrass(Sorghum halepense)but not oat (Avena sativa)by mechanical inoculation. The viruswas partially purifiedby differential centrifugation. An antiserum produced by injection of a rabbit with purified preparation of MM had a titer of 1024 in microprecipitin test and 16 in SDS-agar gel diffusion. In SDS-agar gel diffusion the antiserum formed a precipitin line against purified virus and crude infected Sap, but not against sap from healthy plants. MM reacted strongly with MDMV antiserum and weakly with MS and ScMV antisera, but not with johnsongrass mosaic virus (JgMV) and sorghum mosaic virus (SrMV) antisera in agar-gel diffusion tests. Using antiserum against MM, this virus was quite distinct from MS and ScMV of Dasht-e-Naz in ELISA, DIBA, and SDS-agar gel diffusion tests. Polyacrylamide gel electrophoresis of the virus coat protein showed a single band of about 37 kDa. The genomic RNA had a molecular weight of 3.3x 106 Da in agarose gel electrophoresis.
On the basis of host range, serological relations, and molecular weights of coat proteinand genomic RNA, MM was identified as a MDMV strain.
MM was found to be the most important component of maize infection complex in Dasht-e-Naz by ELISA tests. It was detected in 70 percent of 396 samples of maize with mosaic symptoms tested.

 
Keyword(s): POTYVIRUSES, MAIZE VIRUSES, JOHNSONGRASS VIRUSES, SUGARCANE VIRUSES, SEROLOGY
 
References: 
  • ندارد
 
  Persian Abstract Yearly Visit 60
 
Latest on Blog
Enter SID Blog