Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   August 2001 , Volume 37 , Number 1-2; Page(s) 39 To 50.
 
Paper: 

CHARACTERIZATION OF THE VIRUS CAUSING MOSAIC IN PERSIAN LEEK (ALLIUM AMPLEOPRASUM SSP. PERSICUM)

 
 
Author(s):  DIZAJI ILKHECHI A., IZADPANAH K.A.
 
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Abstract: 

Mosaic disease of Persian leek is common in many fields of Fars and Bushehr provinces in Iran. A mechanically transmissible virus with filamentous particles was isolated from Persian leek in the vicinty of Shiraz and propagated in the same plant under greenhouse conditions. The virus was designated Persian leek mosaic virus (PLMV).
PLMV was purified from leaves of infected Persian leek with polyethylene glycol 6000, sucrose cushion and centrifugation on sucrose-cesium sulfate density gradients. The virus band was removed and concentrated by high-speed centrifugation. Serological tests ELISA, micro precipitin, IEM and agar gel double diffusion were done after antiserum preparation.
Chlorotic local lesions in inoculated leaves of Chenopodium quinoa and systemic mosaic in Onion (Allium cepa) and Persian leek were induced by mechanical inoculation under greenhouse conditions. Garlic (Allium sativum) and onion proved to be natural hosts of PLMV by ELISA.
Normal length of PLMV particles in crude infected plant sap was calculated as 724 nm. Molecular weight of virus coat protein was estimated at 37400 dalton by SDS-PAGE method. The size of virus genome was 3.4x106dalton as determined by electrophoresis in agarose gel.
PLMV was transmitted to healthy Persian leek plants by Aceria (=Eriophyes) tulipae, but not by Aphis gossypii and A. craccivora. The virus was not seed-borne.
Despite its transmission by mites, PLMV appears to be different from other Allium mite-borne viruses in physicochemical properties and host range.

 
Keyword(s): PERSIAN LEEK MOSAIC VIRUS ALLIUM AMPELOPRASUM SSP. PERSICUM, ONION, GARLIC, MITE BORNE VIRUSES
 
References: 
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