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Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   August 2001 , Volume 37 , Number 1-2; Page(s) 1 To 14.
 
Paper: 

RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) ANALYSIS OF SPIROPLASMA CITRI STRAINS ISOLATED FROM CITRUS TREES IN SOUTHERN AND NORTHERN IRAN

 
 
Author(s):  HOSSEINI POUR A., RAHIMIAN H., SAILLARD C., GARNIER M.
 
* 
 
Abstract: 

To determine the genotypic heterogeneity among the Spiroplasma citri strains infecting citrus trees in Iran, S. citri was isolated from fruits of stubborn affected sweet orange and grapefruit trees collected from Fars, Kerman and Mazandaran provinces, during 1997-1999.
Twelve triply-cloned S. citri strains along with two isolates previously cultured from citrus trees in Mazandaran and the type strain of the species (ATCC 27556T) were subjected to restriction fragment length polymorphism (RFLP) analysis. Genomic DNAs of the strains were digested with restriction endonucleases EcoRI and Hind Ill, separated on agarose gels and blotted onto nylon membranes. The blotted membranes were hybridized with digoxigenin labelled 16S rDN A of S. citri (as probe). Hybridization of the EcoRI digested DNAs of all strains, except Shahdad 33, with the 16S rDNA produced two bands measuring 12 and 1.3 Kbp. The Shahdad 33 strain had two bands about 12 and 1.7Kbp in size. Hybridization of the HindIII digested DNAs and the probe, produced only one fragment 4.2Kbp long. Because of the highly conserved nature of the 16S rDNA and its flanking sequences in mollicutes, no remarkable ploymorphism was observed in this part of the genome. However, electrophoretic patterns of the DNAs digested with restriction endonucleases, especially with EcoRI, revealed polymorphism in other parts of the S. citri genome.

 
Keyword(s): CITRUS, STUBBORN, SPIROPLASMA CITRI, RFLP
 
References: 
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