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Paper Information

Journal:   ACTA MEDICA IRANICA   2004 , Volume 42 , Number 3; Page(s) 168 To 171.
 
Paper: 

INHIBITION ASSAY STUDY OF PURIFIED GLUTATHIONE S-TRANSFERASE FROM FASCIOLA HEPATICA AND SHEEP LIVER TISSUE BY HEXACHLOROPHENE

 
 
Author(s):  FARAHNAK A., BROPHY P.M.
 
* 
 
Abstract: 
Glutathione S-transferases (GSTs) are widespread in Fasciola. hepatica parasite and sheep liver tissue. Study of GSTs inhibition assays in F. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in Iran. In this research, the whole extract of F. hepatica and sheep liver tissues were purified and eluted for sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) pattern and GSTs inhibition assay. GSTs inhibition was detected by hexachlorophene as an inhibitor and 1-chloro-2,4-dinitrobenzene (CDNB) as secondary substrate. The purified GSTs from F. hepatica and liver tissue contained comparable components and showed a molecular weight of 26kDa. The inhibitor concentration of hexachlorophene, for the remaining 50% activity (IC50%) of GST enzymes from F. hepatica and liver were graphically calculated, and the results were 0.25 µM and 1 µM, respectively. GSTs of F. hepatica may be more sensitive than sheep liver tissue to hexachlorophene
 
Keyword(s): GLUTATHIONE S-TRANSFERASE , FASCIOLA HEPATICA , PURIFICATION , INHIBITION ASSAY
 
References: 
 
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