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Paper Information

Journal:   JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH   Fall 2003 , Volume 11 , Number 44; Page(s) 1 To 8.
 
Paper: 

THE EFFECT OF HIGH VITAMIN C INTAKE ON CONTRACTILITY OF ISOLATED AORTA INDUCDED BY KCL AND PHENYLEPHERINE IN RATS

 
 
Author(s):  ZAHEDIASL S., PAZHOUHI N., BADVI M., ASAEI R.
 
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Abstract: 

Background: Vitamin C is the most effective water soluble antioxidant substance. Many studies have shown an inverse relationship between the intake of vitamin C and the disorders of cardiovascular system. In this study the effect of vit C intake on contractility of isolated aorta in rats was investigated. Materials and Methods: 60 female wistar rats weighing 180-220g were divided into control (CG) and test groups (TG) as TG1W, TG2W, TG3W, TG4W and TG8W. They received 0.3% vitamin C in drinking water for 1, 2, 3, 4 and 8 weeks respectively. The animals were anesthetized with sodium pentobarbital (50 mg/kg/ip). The chests were opened and the thoracic aortas were removed. The endothelium layer was denuded and a ring of aorta (5-6 mm) was mounted in oxygenated Krebs solution containing organ bath at 37°c and pH=7.4. After a recovery period (90 min), various concentrations of KCl or phenylepherine (phe) were added. The results were reported as mean (SE g/mm2) and compared with ANOVA followed by Turkey's post-Hoc test. A value of P<0.05 was considered significant. Results: There were no significant differences in aortic responses of CG, TG1W and TG2W to KCl and phenylepherine. Potassium chloride in 50, 60 and 75 mmole concentrations in TG3W and TG4W produced tensions of 2±0.2, 2.2±0.2, 2.3±0.2, 1.96±0.2, 1.9±0.1, 1.97±0.1 g/mm2 respectively. In TG8W the same concentrations of KCl produced tensions of 1.5± 1,1.6±0.1, 1.6±0.1 g/mm2. These tensions are significantly (P<0.05) less than the control group. In the aorta, phenylepherine in 1, 5 micromole concentration in TG3W and TG4W produced tensions of 3.2±0.1, 3.5±0.2, 2.7±0.1, 3±0.1 g/mm2 respectively, and in TG8W produced, 2.5±0.2, 2.7±0.2 g/mm2. These tensions are significantly (P<0.05) less than control group. Conclusion: High vitamin C intake in the diet can reduce the aorta contractility in rats.

 
Keyword(s): VIT C, AORTA, RAT
 
References: 
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