Paper Information

Journal:   TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)   2004 , Volume 62 , Number 5; Page(s) 394 To 404.
 
Paper: 

THE EFFECTS OF ASCORBIC ACID AND L-NIL COADMINISTRATION ON LPS INDUCED OXIDATIVE STRESS IN RAT KIDNEY

 
 
Author(s):  PIRMORADI L., KADKHODAEI M., SHAMS S., GHASEMI A.R., MAKI N.
 
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Abstract: 

Background: There are many studies in recent years implicating oxidative stress as the causative factors of many diseases. Lipopolysaccharide (LPS) is suggested to induce septic shock with two mechanisms of burst formation of nitric oxide (NO) by stimulation of inducible NO synthase (iNOS) and oxygen free radical (OFR) formation. Septic shock is usually accompanied with vital organs dis-function such as renal failure. Vitmnin E is the most important fat-soluble antioxidant which protects the biological membranes in oxidative stress. Vitamin C, the most important water-soluble antioxidant acts via scavenging free radicals and regeneration of vitamin E which helps to protect the cells against lipid peroxidation.
Materials and Methods: In this study the changes in renal tissue and plasma vitamin E levels after injection of LPS with and without vitamin C supplementation and L-NIL (a specific iNOS inhibitor) administration were studied. To do this we used five equal groups of rats as: Control, LPS., LPS+L-NIL, LPS+vitamin C, LPS+vitamin C+L-NIL. LDH, BUN, Creatinine, plasma and renal vitamin E levels were measured (with HPLC and UV detection).
Results: LPS markedly decreased vitamin E contents of renal plasma and tissue (P<0.001) while BUN and LDH were significantly increased (p<0.001).L-NIL and vitamin C and their codministration returned all of the parameters back to normal .Coadministration of L-NIL and vitamin  C highly increased vitamin E levels (P<0.001).

Conclusion:These results showthat iNOS inhibition and free radical scavenging prevents vitamin E reduction in septic shock and preserves renal function. 

 
Keyword(s): LPS, ASCORBIC ACID, VITAMIN E, NITRIC OXIDE, KIDNEY
 
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