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Paper Information

Journal:   APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY   February 2004 , Volume 71 , Number 2; Page(s) 141 To 149.
 
Paper: 

COMPARISON OF SEVERAL METHODS FOR DETECTION AND QUANTIFICATION OF ß- EXOTOXIN IN COMMERCIAL BACILLUS THURINGIENSIS PRODUCTS

 
 
Author(s):  MARZBAN R., TAJBAKHSH M.
 
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Abstract: 

The ß -exotoxin (also called thuingiensin and thermo stable toxin) is toxic to human and other animals. Because of vertebrate toxicity, most commercial preparation of B. thuringiensis is composed of subspecies or isolates that do not produce ß -exotoxin. As a condition for registration for pesticide use on food in USA, B. thuringiensis active ingredients must be tested to show the abscense of ß -exotoxin. In this study screening methods for the presence of ß-exotoxin in a product of B. thuringiensis subsp. aizawai (Bill) were based on laboratory bioassay against lepidopteran larvae and High Performance Liquid Chromatography (HPLC). The bioassay and HPLC assay results indicated that the ß -exotoxin was presence in the product of B. thuringiensis subsp. aizawai (Bill). Bioassay was showed to be more sensitive than High Performance Liquid Chromatography (HPLC). Bioassay can be used to detect and qualify B. thuringiensis ß -exotoxin and can be a useful tool for quality control.

 
Keyword(s): BACILLUS THURINGIENSIS SUBSP. AIZAWAI, BETA-EXOTOXIN, HPLC, BIOASSAY, BILL
 
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