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Paper Information

Journal:   ARCHIVES OF IRANIAN MEDICINE   January 2001 , Volume 4 , Number 1; Page(s) 10 To 13.
 
Paper: 

INACTIVATION OF POLIOVIRUS TYPE-1 AND HSV-1 IN HUMAN COAGULATION FACTOR VII CONCENTRATE BY PASTEURIZATION

 
 
Author(s):  REZVAN H., NASIRI S., MOUSAVI K.
 
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Abstract: 
Background-Pasteurization is now available to treat products derived from plasma to inactivate transfusion-transmitted enveloped and non-enveloped viruses. Objective-This study was performed to assess the capacity and efficacy of virus inactivation by pasteurization in human coagulation factor VII concentrate with two model viruses including poliovirus type 1 (non-enveloped) and herpes simplex virus type 1 (enveloped). Methods-The viruses were added to the stabilized factor VII (70% sucrose, 20% glycine) and pasteurized for 10 h at 60° C. Viral infectivity was tested in vitro on cell culture (Vero) using standard microtitration assays and the virus titer was expressed as log10 TCID50/ml. Results-On completion of the pasteurization procedure, 6.3 log of poliovirus type 1 and 5.8 log of HSV-1 were inactivated. Recovery of factor VII activity using sucrose/glycine as stabilizer was 80%. Other stabilizers showed lower recovery of factor VII and were less suitable. Conclusion-Viral inactivation by pasteurization procedures destroys both enveloped and non-enveloped viruses and can be applied to production of factor VII from plasma with relatively good recovery and can improve the safety of replacement therapy in hemophilia patients with inhibitors.
 
Keyword(s): FACTOR VII • VIRAL INACTIVATION • PASTEURIZATION
 
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