Paper Information

Journal:   JOURNAL OF ENVIRONMENTAL STUDIES   September 2004 , Volume 30 , Number 34; Page(s) 7 To 14.
 
Paper: 

ISOLATION OF PETROLEUM DEGRADING BACTERIA FROM PERSIAN GULF AND THE STUDY OF THE PH EFFECT ON OIL MINERALIZATION

 
 
Author(s):  EBRAHIMIPOUR GH.H., ABOU ALHASANI SOURAKI A.
 
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Abstract: 
Two barcteria, capable of petroleum biodegradation were isolated from Persian Gulf seawater. Both strains (designated as PGOl and PG02) were marine halophile, strictly aerobic, motile, gram-positive short rods. During growth (in 250 ml erlen meyer containing 100 ml mineral base medium with 1% crude oil as sole carbon source, crude oil medium), under shaking condition (150 rpm), both strains produced biosurfactant which emulsified petroleum and bacterial cells were found to attach to generated oil droplets. The optimum pH for both isolated growth in the crude oil medium was 8-8.4, similar to the pH of seawater. Both isolates could grow in mollasses medium (1%) but biosurfactant production in this hydrophilic substrate was not observed.
 
Keyword(s): CRUDE OIL BIODEGRADATION, PERSIAN GULF, BIOSURFACTANT, PH
 
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