Paper Information

Journal:   IRANIAN POLYMER JOURNAL (ENGLISH)   November-December 2003 , Volume 12 , Number 6; Page(s) 491 To 495.
 
Paper: 

SYNTHESIS OF MACROPOROUS POLYMER CARRIER AND IMMOBILIZATION OF PAPAIN

 
 
Author(s):  DING L.*, YAO Z., LI T., QIU M., YUE Q., CHAI J.
 
* Hebei College of Continuing Medical Education, Baoding, China
 
Abstract: 

A macroporous resin microbeads, methyl methacrylate-divinyl benzene copolymer was synthesized by radical suspension polymerization with divinylbenzene in the presence of a pore –creating agent, petroleum ether. They have big specific surface area and a large of porous was covered on all surface of the resin. This macroporous polymer carrier was aminated by hydrazine hydrate, then produce a large of amino groups to the carrier. Papain was immobilization on the porous polymer carrier by the glutaraldehyde cross-linking method or diazo –coupling method.The factors involved with the activity recovery of the immobilized papain and the enzymic properties of the resulting immobilized papain were studied in comparison with free papain, for which casein was chosen as a substrate. The results show that the activity recovery of immobilized papain can reach 60.1%. At the same time, the stability, resistance against inhibition, the Michaelis constant Km, and reusability of the immobilized enzyme were also investigated. The results indicated that the immobilized papain by this reached had not only higher activity recovery, but also remarkable stability, better reusability and environmental adaptability than free papain.

 
Keyword(s): IMMOBILIZATION, PAPAIN, IMMOBILIZED ENZYME, MACROPOROUS POLYMER CARRIER, ENZYME ACTIVITY
 
References: 
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