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Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   December 2002 , Volume - , Number 54; Page(s) 1 To 15.
 
Paper: 

CHARACTERIZATION OF AVIAN PASTEURELLA MULTOCIDA ISOLATES BY PROTEIN PROFILES AND RESTRICTION ENZYME ANALYSIS OF CHROMOSOMAL DNA

 
 
Author(s):  JABARI A.R.*, SAHAREE A., ESMAEILI F.
 
* AEROBIC VETERINARY BACTERIAL VACCINES RESEARCH & PRODUCTION DEPT., RAZI VACCINE & SERUM RESEARCH INSTITUTE, TEHRAN, IRAN
 
Abstract: 

The protein profiles of 25 avian Pasteurella multocida isolates were studied by discontinuous polyacrylamide gel electrophoresis. The Coomassie blue stained gel showed that each whole cell protein lysate of P multocida contained over 30 protein bands ranging from 24 to 174 KDa. Majority of protein bands was located in the mid section of the gel between 30 and 97 KDa. All isolates were similar in the majority of protein bands. The major difference between proteins patterns of isolates was revealed in the position of a major outer membrane protein band (H Protein) presented in the 34-38 KDa regions. According to molecular mass, the isolates were classified in three protein profiles. Restriction enzyme analysis (REA) of chromosomal DNA by HpaII distinguished six groups. Two of which contained a single isolate. The Iranian vaccine strain (PM1030) and thirteen field isolates belonged to REA type I. There was not any relationship between serotype, protein profile and REA pattern of the isolates.

 
Keyword(s): POSTEURELLA MULTOCIDA, POULTRY, PROTEIN PROFILE, RESTRICTION ENZYME ANALYSIS
 
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