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Paper Information

Journal:   ROSTANIHA   2004 , Volume 5 , Number 3; Page(s) 25 To 36.
 
Paper: 

PART ONE: PHYLOGENETIC STUDIES USING ITS SEQUENCES

 
 
Author(s):  ZARE R., GAMS W.
 
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Abstract: 
Results of ITS analyses Variation of amplicon length: The total length ofITS-I-5.8S-ITS-2 across Verticillium s. l. varied from 476 bp in IMI 130213 (v. nubilum, clade A) to 558 bp in CBS 248.83 (V. suchlasporium var. catenatum, clade D). The 5.8S gene is very conserved both in sequence and in length, ranging from 158 bp in Cephalosporium curtpes var. uredinicola CBS 154.61 to 205 bp in V. lamellicola CBS 116.25. Rotiferophthora species, which are close to clade D, had a comparatively longer 5.8S gene (200-207 bp). Size variation was much greater in the ITS region: for ITS-l from 126 bp (in V. tricorpus) to 189 bp (in V. antillanum) and ITS-2 from 145 bp (in V. zeosporum) to 188 bp (in V. suchlasporium var. catenatum). Rotiferophthora species had relatively long ITS-l (204-215 bp) and ITS-2 sequences (156-186 bp).
Sequence divergence: Sequence variations were calculated using P AUP version 4.0b4a (SWOFFORD, 1998). Up to 60% sequence variation was observed in the ITS region across Verticillium s. I., rendering alignment problematic, particularly of the species included in Clade A. Only the 5.8S gene was highly conserved (Table I-Ill), serving as a basis for the alignment. Average pairwise sequence divergence of the ITS region within Verticillium s. l. was 10-36% in ITS-l and 5-43% in ITS-2, while these values were much smaller for the 5.8S gene, ranging from 2 to 10% (Table I-Ill). The highest differences were found between Verticillium clade A and the remaining clades, indicating their distant relationship. Rotiferophthora and clade D are closest to each other, with only 8-16 and 8-21% sequence difference in their ITS-l and ITS-2, respectively. ...
 
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