Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   June 2003 , Volume - , Number 55; Page(s) 55 To 62.
 
Paper: 

STANDARDIZATION OF ANTI-DNA ELISA KIT IN RAZI INSTITUTE

 
 
Author(s):  MOUSAVI T.*, AKHAVIZADEGAN M.A., ASADI N.
 
* Biotechnology Dept., Razi Vaccine & Serum Research Institute, Tehran, Iran
 
Abstract: 

In order to develop an ELISA kit for detection of anti-DNA antibodies several procedures were examined. In this study the best method for native DNA preparation, solid phase preparation, and formulation of buffers, design of test performance, and a storage condition of the kit components was introduced. DNA extraction was performed by phenol procedure and purified one more time to lose protein impurities. To prepare the coated micro plate, a percoating stage using poly-L lysine to enhance DNA attachment and an extra post-coating stage for neutralizing its negative charge were applied. To determine the sensitivity and specificity, 120 serum samples were simultaneously tested with the developed and commercial kits. The results indicate that sensitivity and specificity of the developed kit is 97% and 100% respectively with 99% accuracy and 5% coefficient of variations. Moreover periodic examinations on kit components reveal that the kit is stable at least one year in 4°C without diminished quality.

 
Keyword(s): ELISA, ANTI-DNA ANTIBODY, SLE
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 130
 
Latest on Blog
Enter SID Blog