Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   June 2003 , Volume - , Number 55; Page(s) 19 To 28.
 
Paper: 

IMMUNOGENICITY AND EFFICACY OF BACULOVIRUS DERIVED GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS TYPE-1 IN MICE

 
 
Author(s):  SOLEYMANJAHI H., ROUSTAEI M.H.*, RASSAEE M.J., MAHBOUDI F., BAMDAD T.
 
* Virology Dept, Faculty of Medical Science,Tarbiat Modares University, Tehran, Iran
 
Abstract: 

The recombinant glycoprotein D (gD) of herpes simplex virus type- 1 (HSV-1) in baculovirus expression system was produced. Spodoptera Frugiperda cell, clone 9 (Sf9) was cultured in modified Graces medium and inoculated with 57 multiplicity of infection of HSV-1 recombinant baculovirus carrying gD gene. Inoculated cells were harvested 96h postinoculation and treated with 3[(3-cholamidopropy1)dimethylainmonio]-1-propanesulfonate (CHAPS) and phenylmethylsulfonyl fluoride (PMSF) and sonicated at 20KHZ for 5 times. A western blotting NNas developed and applied to detect the prepared protein. Three groups of BALB/c mice each of 7 mice were inoculated with the recombinant gD, Sublethal dose of challenge virus (104.5 TCID50) and PBS respectively. All inoculated mice were challenged with 10MLD50 (106.5 TCID50) of the wild HSV-1. All mice who had received the recombinant gD survived while 14.3% and 71.5% of mice inoculated respectively with either Sublethal dose of the virus or PBS died.

 
Keyword(s): HERPES SIMPLEX VIRUS GD, RECOMBINANT PROTEIN, WESTERN BLOTTING, IMMUNIZATION
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 102
 
Latest on Blog
Enter SID Blog