Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   June 2003 , Volume - , Number 55; Page(s) 11 To 18.
 
Paper: 

DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION

 
 
Author(s):  KARGAR MOAKHAR R., GHOREYSHI S.A.*, SADEGHI M.R., MORSHEDI D., MASOUDI S., POURBAKHSH S.A.
 
* Animal Biotechnology Dept., National Research Center for Genetic Engineering and Biotechnology, Engineering and Biotechnology,
 
Abstract: 

Based on primers from gI glycoprotein, a polymerase chain reaction (PCR) assay was optimized for detection of bovine herpes virus type 1 (BHV-1). A 468bp DNA fragment of nine isolates was amplified. The PCR products were detected by ethidium bromide staining after gel electrophoresis and confirmed by sequencing. The nucleotide sequence alignments revealed a highly conserved region in gI gene within the isolates. Results suggest that PCR can be used as a reliable diagnostic tool for rapid detection of BHV-1 infections.

 
Keyword(s): BOVINE HERPESVIRUS 1, GI GENE, POLYMERASE CHAIN REACTION, IRAN
 
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