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Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   June 2003 , Volume - , Number 55; Page(s) 11 To 18.
 
Paper: 

DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION

 
 
Author(s):  KARGAR MOAKHAR R., GHOREYSHI S.A.*, SADEGHI M.R., MORSHEDI D., MASOUDI S., POURBAKHSH S.A.
 
* Animal Biotechnology Dept., National Research Center for Genetic Engineering and Biotechnology, Engineering and Biotechnology,
 
Abstract: 

Based on primers from gI glycoprotein, a polymerase chain reaction (PCR) assay was optimized for detection of bovine herpes virus type 1 (BHV-1). A 468bp DNA fragment of nine isolates was amplified. The PCR products were detected by ethidium bromide staining after gel electrophoresis and confirmed by sequencing. The nucleotide sequence alignments revealed a highly conserved region in gI gene within the isolates. Results suggest that PCR can be used as a reliable diagnostic tool for rapid detection of BHV-1 infections.

 
Keyword(s): BOVINE HERPESVIRUS 1, GI GENE, POLYMERASE CHAIN REACTION, IRAN
 
 
References: 
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+ Click to Cite.
APA: Copy

KARGAR MOAKHAR, R., & GHOREYSHI, S., & SADEGHI, M., & MORSHEDI, D., & MASOUDI, S., & POURBAKHSH, S. (2003). DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION. ARCHIVES OF RAZI INSTITUTE, -(55), 11-18. https://www.sid.ir/en/journal/ViewPaper.aspx?id=112



Vancouver: Copy

KARGAR MOAKHAR R., GHOREYSHI S.A., SADEGHI M.R., MORSHEDI D., MASOUDI S., POURBAKHSH S.A.. DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION. ARCHIVES OF RAZI INSTITUTE. 2003 [cited 2021May15];-(55):11-18. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=112



IEEE: Copy

KARGAR MOAKHAR, R., GHOREYSHI, S., SADEGHI, M., MORSHEDI, D., MASOUDI, S., POURBAKHSH, S., 2003. DETECTION OF DIFFERENT IRANIAN ISOLATES OF BOVINE HERPES VIRUS TYPE-1 (BHV-1) USING POLYMERASE CHAIN REACTION. ARCHIVES OF RAZI INSTITUTE, [online] -(55), pp.11-18. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=112.



 
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