Paper Information

Journal:   ARCHIVES OF RAZI INSTITUTE   June 2003 , Volume - , Number 55; Page(s) 1 To 10.
 
Paper: 

FIELD STUDY OF INFECTIOUS BRONCHITIS VIRUS IN BROILER USING TYPE-SPECIFIC RT-PCR

 
 
Author(s):  SHAHRIARI ALI REZA, SEYFIABAD SHAPOURI M.R., NOURI A.*
 
* Poultry Clinic, Veterinary School of Shiraz University, Shiraz, Iran
 
Abstract: 

The molecular detection of avian infectious bronchitis virus by use of RTPCR and multiplex nested PCR in Fars province of Iran was investigated. Detection has been done on tracheal swab samples of 30 broiler flocks in age of 7-8 weeks. Flocks were selected from must broilers rising regions of the province. Detection was performed by primers specific for Massachusett, 4/91 and D274 serotypes. In this study 16 samples were positive for IBV 4/91 typeand 1 for Massachuset type. One swab sample showed a mixed infection of those serotypes. D274 serotype has not been detected in this study. This is the first report of the presence and a prevalence of IBV type 4/91 in Fars province.

 
Keyword(s):  INFECTIOUS BRONCHITIS VIRUS, BROILER, RT-PCR, IRAN
 
References: 
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