Background and Objectives: Appropriate culture media and environment for embryonic cell growth considered to be major factors for generation of an embryo in vitro. In this study two embryonic growth condition of GI_III and Ham's F- 10+ Maternal Serum (MS) %10 were compared.
Materials and Methods: This investigation was prospectively performed on 100 infertile couples that were treated by IVF-ICSL the participants were divided into two equal groups. Ovules obtained from first group were treated with Ham's F₁₀+ Maternal Serum %10 culture media and the second group with GI_III. The variables affecting ovule and embryonic growth were measured, and collected data were analyzed by SPSS.
Results: The mean of age for tested ladies was 28.01 and the mean for duration of infertility was 6.87 years. The number of pronucleous with moderate quality were 2.14 in-group 1 and 3.22 in-group II (P=0.023). The number of embryo with three cells were 1.26 and 0.54 and for grade B embryonic cell were 1.18 and 1.78 and grade C embryonic cell were 1.08 and 0.56 in group 1and II respectively (p=0.29). The mean of grade B blastomer in GI_III media was more than Ham's F₁₀+ Maternal Serum 10%. Comparing the means; the mean of grade B blastomers in Gl_III media was shown to be more than Ham's F₁₀+ Maternal Serum 10%.
Conclusion: All of the findings showed that GI_III cultural condition is more effective than Ham's F₁₀ and the maternal serum as a supplement has no considerable impact.