The purpose of this research was to study the acute effect of single and double session of exhaustion progressive exercise on the immunity factors in activity girls. For this reason, 21 healthy girl student of physical education with age 20.77±1.93 years, weight 57.33±5.54kg, height 162.17±5.62cm and aerobic capacity 41.07±5.11 ml/kg/min voluntary participated in this study, had been selected and randomly divided in 3 groups: one experimental group (2 session of exercise in a day, n=8), two experimental group (1 session of exercise in a day, n=7) and control group (n=6). Blood samples were taken in 2 stages: 1. 24 h before exercise for detecting of baseline of leucocytes, lymphocytes and neutrophiles (pre- test condition) 2. After exercise in the afternoon (post-test condition). ANOVA test, paired t-test and Tukey test were used to Statistics analysis for data in signification level loss 0.05 (p£0.05) to used SPSS13. The findings of this study indicated that: The count of leucocytes, lymphocytes and neutrophiles was significantly increased in one and two experimental group relative to baseline and comparison with control group (p£0.05). In addition there was no significant difference between one and two, experimental groups (p>0.05). In general the result of this study was expressed that 2 session of exercise in comparison of with 1 session of exercise group in day was a few effect on number of leucocytes, lymphocytes and neutrophiles of active girls. Therefore, the results of this study suggest that more research was achieved in field.