The aim of present study was to assess the effect of one period of regular walking on the level of general, trait and state anxiety of pregnant women in the 2nd and 3rd trimester of pregnancy. Using a quasi-experimental field approach some 40 pregnant, two experimental and control groups, were selected. At first all subjects answered to Cattle Anxiety Questionnaire (CAQ), then after presenting a special walking program, all subjects answered to the CAQ again. There was a significant difference between general, trait and state anxiety levels in experimental and control groups (P<0.05). There wasn’t any significant difference in difference between means of the pre-/post-tests of the general and trait anxiety, while the difference was seen in the state anxiety of the test groups (P<0.05).