Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   SPRING 2008 , Volume 10 , Number 1 (37); Page(s) 33 To 40.
 
Paper: 

EFFECT OF STAUROSPORINE ON NEURAL DIFFERENTIATION OF CD133+ UMBILICAL CORD BLOOD CELLS

 
 
Author(s):  FAGHIHI FAEZEH, SOLEYMANI MEHRANJANI M., ZARE MEHRJERDI N., BAHARVAND HOSSEIN*
 
* STEM CELL DEPARTMENT, CELL SCIENCES RESEARCH CENTER, ROYAN INSTITUTE, ACECR, TEHRAN, IRAN
 
Abstract: 

Objective: CD133+ umbilical cord blood cells were identified as a hematopoietic stem cell which has the capacity for extensive self-renewal and differentiation. The aim of this study was to identify the effect of staurosporine (STS), a well-known protein kinase inhibitor on differentiation of CD133+ cells into neural cells.
Materials and Methods: CD133+ cells were enriched by immunomagnetic beads from human mononuclear cells of umbilical cord blood and the purity of higher than 94% was achieved by flowcytometry. Induction of differentiation was performed by addition of STS (12.5, 25, and 50nM). The differentiated cells were evaluated by immunofluorescence and RT-PCR for neuron-specific proteins and transcripts.
Results: STS-treated CD133+ cells expressed mRNA transcripts for neuron-specific neurofilament protein (NFM), and several basic helix-loop-helix (bHLH) transcription factors important for early neurogenesis, including Otx2, Wnt1, and Hash1. The structural proteins characteristics of neurons including
b-tubulinlll and Microtubule-Associated Protein-2 (MAP-2), were shown by immunocytochemistry. STS-treated CD133+ cells also expressed the astrocyte-specific marker, glial fibrillary acidic protein (GFAP) by immunofluorescence.
Conclusion: The human cord blood-derived CD133+ hematopoietic stem cells could differentiate into neural cell types of neuron-like cells and astrocytes by STS treatment.

 
Keyword(s): STAUROSPORINE, CD133+ CELLS, UMBILICAL CORD BLOOD, NEURAL DIFFERENTIATION
 
References: 
 
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