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Paper Information

Journal:   PAJOUHESH-VA-SAZANDEGI   SUMMER 2007 , Volume 20 , Number 2 (75 IN NATURAL RESOURCES); Page(s) 173 To 178.
 
Paper: 

IN VITRO MICRO PROPAGATION OF DENDEROSTELLERA LESSERTII VAN TIEGH

 
 
Author(s):  GHAMARI ZARE A., GHORBANLI MAH LAGHA, HOSSEINI SHEYDA, SHAHRZAD SH.
 
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Abstract: 

Dendrostellera lessertii Van Tiegh has been distributed around rangeland of Iran as an endemic plant species. The plants were not interest for animal and poultry, because of un-palatability. It’s leaves extraction contains poisonous chemical materials which prevents cancer cells progression. The pure extraction D. lessertii’s leaves effect on alkaline phosphates’ enzyme of four types of human cancer cells. The seeds of D. lessertii were collected from ranglands of Esfahan and Markazi provinces of Iran. Seeds, after 42 hours being under running water, were disinfect with NaOCl (1%) for 5 min and cultured on medium treatments in a complete randomized block designed and the means of them were compared with Duncan test at a=5%. The best treatment for shooting was MS medium contains 1/2 KNO3, BAP (0.3 mg/l), 2ip (0.3 mg/l) and IBA (0.01 mg/l). Rooting of shoots carried out in 15 culture medium treatments (root’s hormone, semi-solid or liquid substrate and concentration of sucrose and macro elements). The best treatment for rooting was 1/3 MS semi-solid contains 1/3 macro elements, 1/2 KNO3 and 1/3 Sucrose, with the IBA (0.5 mg/l). Rooted plantlets translated to puts contains humus, perlit and sand with the same amount (1:1:1) in germinator for adaptability.

 
Keyword(s): MEDICINAL PLANT, CANCER CELLS, MICRO PROPAGATION, SHOOTING, ROOTING AND ADAPTABILITY
 
References: 
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