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Paper Information

Journal:   JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)   WINTER 2008 , Volume 17 , Number 66/1 (BIOLOGY ISSUE); Page(s) 5 To 12.
 
Paper: 

SINKING REASONS IN CYSTS OF ARTEMIA URMIANA WITH EMPHASIS ON SHELL STRUCTURE

 
 
Author(s):  HAJI ROSTAMLOU M.*, EMADI H., SEPAHDARI A.A.F.
 
* BIOLOGY DEPARTMENT, MARAND BRANCH, ISLAMIC AZAD UNIVERSITY, MARAND, IRAN
 
Abstract: 

Artemia is a small crustacean that adapted to live in brine water and in unfavorable condition produce an embryo in gastrula state with tertiary shell that named cyst. The main source of Artemia in Iran is Urmia Lake, existence of two different groups Artemia urmiana cysts with different ability in buoyancy cased. This study was done to reveal structural difference between buoyant and sink cysts, role of shell layer in buoyancy or sinking, and relation between depth difference and shell layers thickness. Cysts of Artemia urmiana after collection from surface and bed for electromicroscopy fixed in glutaraldehyde and osmium tetroxide and dehydrated by graded series of ethanol and propylene oxide. Then, they embedded in resin (Agar 100) and 70-80 nm sections, and colored in two stages by uranyl acetate and lead citrate. Comparison between sections of surface and bed samples showed some differences in thickness of alveolar, 4.5 to 16mic and fibrous, 5.5 to 0.65mic Results showed structural differences, revealed shell layers are effective in buoyancy or sinking of cysts and against previous ideas about non-relation between buoyancy and shell layers thickness confirm that cysts set in different depths based on their thickness specially alveolar layer.

 
Keyword(s): ARTEMIA URMIANA, SINKING, CYST, SHELL STRUCTURE, ELECTROMICROSCOPY
 
References: 
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