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Paper Information

Journal:   JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS)   MARCH 2008 , Volume 5 , Number 1; Page(s) 115 To 121.
 
Paper: 

APPLICATION OF ARSENAZO III FOR THE POLAROGRAPHIC DETECTION OF PROTEINS

 
 
Author(s):  SUN W.*, ZHAO N., XU B., WANG M.N., JIAO K.
 
* COLLEGE OF CHEMISTRY AND MOLECULAR ENGINEERING, QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY, QINGDAO 266042 P.R. CHINA
 
Abstract: 
In this paper, a diazo dye of arsenazo III (AAIII) was selected as a new electrochemical probe for the determination of proteins. In Britton-Robinson (B-R) buffer solution of pH 2.4, AAIII had a sensitive second order derivative linear sweep voltammetric reductive peak at -0.39 V (vs. SCE). After the addition of human serum albumin (HSA) into AAIII solution, an interaction was taken place in the mixed solution and a biosupramolecular complex was formed, which resulted in the decreased reductive peak currents of AAIII. Based on the observed decrease in peak current, a sensitive electrochemical method was proposed for the determination of different proteins such as HSA, bovine serum albumin (BSA) and bovine hemoglobin (BHb). The optimal conditions for the interaction and the interfering effects of coexisting substances on the detection were investigated. The proposed method was successfully applied to the determination of HSA in synthetic samples with the recoveries in the range of 99.13 -100.50%. The stoichiometry of HSA-AAIII biocomplex was calculated by voltammetric data with a binding number of 2 and a binding constant of 7.53 × 109.
 
Keyword(s): ARSENAZO III, HUMAN SERUM ALBUMIN, LINEAR SWEEP VOLTAMMETRY, INTERACTION, PROTEIN ASSAY
 
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