Paper Information

Journal:   IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)   2007 , Volume 37 , Number 6; Page(s) 1141 To 1149.
 
Paper: 

IN VITRO MASS PROPAGATION OF GRAPEVINE (VITIS. VINIFERA L.) FROM FRAGMENTED SHOOT TIP VIA DIRECT ORGANOGENESIS

 
 
Author(s):  SALAMI S.A.R.*, EBADI ALI, ZAMANI Z.A., GHASEMI MARYAM
 
* UNIVERSITY COLLEGE OF AGRICULTURE & NATURAL RESOURCES, UNIVERSITY OF TEHRAN
 
Abstract: 
Through a simple and rapid in vitro propagation method while using fragmented shoot apices and via direct organogenesis, plants of grapevine (Vitis vinifera L.) cv.
'Bidaneh Sefid' were propagated. Fragments were obtained from individual apices planted in liquid medium of Murashige & Skoog (1962) supplemented with 2.0 mg/l BA and 30 g/l sucrose. To determine the effects of BA concentration on shoot multiplication, 4 week primary leaf-like structures obtained from liquid medium, were subcultured in basal solid medium containing 6.5 g/l agar, 30 g/l sucrose as well as different growth regulators (0.5BA, 1.0BA, 2.0BA, 0.5BA+0.1 IBA & 1.0 BA+O.IIBA) mg/J. They were transferred to solid medium after 3 and 6 weeks. Results showed significant differences among treatments, as regards the number of finally obtained shoots. In an evaluation of the media, and the effect on the number of shoots, MS medium supplemented with 2 mg/l BA produced mean shoot numbers of 21 and 102.5 after 3 and 6 weeks respectively, (the highest number of shoots), and was chosen as the best hormone composition for continuing proliferation through plantlets. Shoots grown in this medium were sub cultured regularly and plantlets obtained were rooted in a hormone-free Murashige and Skoog (1962) medium in half concentration while 20 g/l sucrose. Plants were then, acclimatized and transferred to greenhouse successfully. This in vitro propagation method has the potential to produce a large number of plants from a single shoot tip in a short period of time. It also has the commercial potential in mass clonal propagation of grapevine. Results indicate that through this method one can produce numerous healthy plants from one single shoot tip during only 20 weeks, and through just two sub culturing.
 
Keyword(s): GRAPEVINE, IN VITRO CULTURE, BIDANEH SETID, DIRECT ORGANOGENESIS, FRAGMENTED SHOOT TIP
 
References: 
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