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Paper Information

Journal:   IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)   2007 , Volume 37 , Number 6; Page(s) 1115 To 1120.
 
Paper: 

AN INVESTIGATION OF DIFFERENT TREATMENTS ON STARVED RUMEN BACTERIA AND THEIR EFFECTS ON SHIFT-UP GROWTH

 
 
Author(s):  MOHARERI A.*
 
* FACULTY OF AGRICULTURE, UNIVERSITY OF SHAHRE KORD
 
Abstract: 
Rumen liquor was taken from a mature lactating dairy cow with permanent rumen cannula, fed finger millet (Eleusine coracana) straw as a roughagead libitum and mixed commercial concentrate to meet its energy requirements. Bacterial cells were separated using centrifugation of rumen liquor and kept on phosphate buffer for a period of thirty days. Two glucose concentrations as well as two types of isoacids (valeric and isovaleric) along with glucose were employed for shifting-up growth after starvation. A roll tube method was used for maintenance and cultivation of bacteria while the changes of turbidity were employed as an indicator for either reduction or increase of bacterial mass in various times. Results indicated that bacterial cells could remain alive in the buffer medium without any nutrient for duration of 30 days, but after this period they need a long time for shifting-up growth as compared with the normal conditions. The time needed for shifting-up growth is related to the kind of nutrient in the medium. In low glucose concentration (19.7 mmol/ml of media) the shifting-up growth started after 12 hours of the solution injection into the tubes while logarithmic growth is carried out during the next 12 hours. But in high glucose concentration (39.42 mmol/ml of media) logarithmic growth started after 18 hours of solution injection and was kept on through the next 29 hours along with higher bacterial concentration. Isovaleric acid (15 mmol + 13.86 mmol glucose per ml of media) in comparison to valeric acid (17 mmol + 13.86 mmol glucose per ml of media) indicated that bacteria in Isovaleric acid treatment need less time (two hours) for going to the shifting-up growth stage and more time (two hours) to reach the stationary phase. In addition, bacteria concentrations in valeric acid treatment were higher than those in isovaleric acid ones (P< 0.05).
 
Keyword(s): RUMEN BACTERIA, STARVATION, SHIFT-UP GROWTH
 
References: 
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