Paper Information

Journal:   IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)   2007 , Volume 37 , Number 6; Page(s) 1103 To 1113.
 
Paper: 

REGENERATION AND PROLIFERATION OF SHOOTS FROM VARIOUS TISSUE EXPLANTS AND A COMPARISON OF SHOOT ROOTING UNDER IN VITRO AND GREENHOUSE CONDITIONS IN SIXTEEN COMMERCIAL CULTIVARS OF AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL.)

 
 
Author(s):  SHAJIEI K.*, NADERI R., KHALIGHI A., TEHRANIFAR A.
 
* UNIVERSITY COLLEGE OF AGRICULTURE & NATURAL RESOURCES, UNIVERSITY OF TEHRAN, KARAJ
 
Abstract: 
Regeneration capacity and shoot multiplication rates of different tissue explants including leaf lamina, petiole, petal and inflorescence of sixteen commercial cultivars of African violet were compared under in vitro and under greenhouse conditions. MS standard culture medium plus 30 gr/l of sucrose, 1 mg/l IBA and 3 mg/l BA were used in all explants cultures. The highest and the lowest number of regenerated shoots were obtained from leaf and inflorescence explants (34 and 8 shoots within 8 weeks) respectively.MS standard culture medium plus 30 gr/l of sucrose, and the amounts of 0, 0.5, I, 2 and 3 mg/l of both IBA and NAA were used for shoot cutting cultures while peat and perlite were compared in rooting of shoots under greenhouse conditions.
Rooting and regeneration of shoots were increased using 2 and 3 mg/l of IBA, while dedifferentiation and callus induction were observed in media containing 2 and 3 mg/l of NAA. No callus was produced in the no growth regulator containing culture media (control) where the shoots were rooted faster as compared with the hormone-treated media. Perlite was shown to be a better medium for rooting of shoots as compared with either peat or in vitro culture media.
 
Keyword(s): AFRICAN VIOLET, SHOOT MULTIPLICATION RATE, NAA, IBA, PEAT AND PERLITE
 
References: 
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