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Paper Information

Journal:   IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)   2007 , Volume 37 , Number 6; Page(s) 1001 To 1015.
 
Paper: 

AN IDENTIFICATION OF CLONAL LINEAGES IN POPULATIONS OF MAGNAPORTHE GRISEA USING REP-PCR AND A STUDY OF THEIR DISTRIBUTION IN GUILAN PROVINCE, IRAN

 
 
Author(s):  JAVAN NIKKHAH M.*, HEJAROUD GH.A., MCDONALD B., SHARIFI TEHRANI A., OKHOVAT S.M.
 
* UNIVERSITY COLLEGE OF AGRICULTURE AND NATURAL RESOURCES, UNIVERSITY OF TEHRAN
 
Abstract: 

One hundred and seventy monoconidial isolates of Magnapor the grisea, collected from different areas of Guilan province, were examined using rep-PCR genomic fingerprinting for charcterization of genetic diversity and for identification of clonal lineages in populations of the fungus. Isolates were recovered from ten common rice cultivars in different rice growing areas during 1997-1999. Two outwardly directed primer sequences designated from terminal inverted repeats of Pot2 (a repetitive transposable element specifically found throughout fungal genome) were used in this experiment. Fungal genomic DNA was extracted using phenol/ chloroform method for long PCR conditions, including increased extention time (10 min) and higher pH (9.2) used to amplify sequences lying between Pot2 elements. It generated variable length fragments ranging from 550 bp to longer than 5 kb. Each isolate was subjected to DNA fingerprinting, and both Pot2 lineage (isolates with  ³70% band similarity), and haplotype (isolates which had DNA fingerprints which differed by 5 to 30%) frequencies were determined. Phenetic analysis differentiated five distinct Pot 2 fingerprint lineages designated A, B, C, D and E. Among 170 isolates, 14, 2, 4, 3 and 18 haplotypes were identified within lineages A, B, C, D, and E, respectively. Lineage E with frequency of appromixately 57.6% of all isolates made the most common and largest fingerprint group, recovered from all rice cultivars sampled, especially local cultivar Tarom, and was distributed throughout Guilan. Haplotype F6, the most common haplotype within lineage F, was recovered from almost all sampling sites. Lineage A, the second most common lineage, with 29% frequency, was found mainly in the central and western parts of Guilan. Haplotype AI, the most common haplotype within lineage A, was found in most sampling sites in the western province. LineageA occurred at highest frequency on a susceptible local cultivar, Binam. Three other lineages totally exhibited appromixately 9.5% frequency. On the contrary to results of pathogenicity tests and number of pathotypes identified in the past, this investigation indicated a low level of genetic diversity in the M grisea population based on molecular method in Guilan. The result is similar to that reported in other countries. It is likely that the main reasons for a low level of diversity are a lack of sexual recombination, occurence of asexual reproduction and a short growing season for rice in Guilan province. By asexual reproduction, clonal propagation occures in M grisea populations and is not affected by high variations.

 
Keyword(s): RICE, BLAST, MAGNAPOR THE GRISEA, CLONAL LINEAGE, HAPLOTYPE, DISTRIBUTION
 
References: 
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