Paper Information

Journal:   IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)   2007 , Volume 37 , Number 6; Page(s) 993 To 1000.
 
Paper: 

MICROSCOPIC DETERMINATION OF GENERATIVE AND NUCELLAR EMBRYO FORMATION TIME AND THEIR DEVELOPMENT IN FIVE CITRUS ROOTSTOCKS

 
Author(s):  GHASEMI MALEK, EBADI A.*, ZAMANI Z.A.
 
* UNIVERSITY COLLEGE OF AGRICULTURE & NATURAL RESOURCES, UNIVERSITY OF TEHRAN
 
Abstract: 
In this trial the stages of zygotic and adventitious embryo development and the time of polyembryony were studied via microscopical analysis in five citrus rootstocks (Sour Orange, Flying Dragon, Citromelo, Citrange and Shell Mahalle). The fruits were harvested from each genotype at 35, 50, 65, 80, 95, 110 and 125 days after pollination (DAP). Diameter and weight of fruits were measured, then seeds were extracted and fixed in FAA. After cross section with Paraffin method and staining with Safranin- Fast green the number of embryos per seed were determined. The experimental design was RCD with three replications, each replication being consisted of ten seeds. Results showed that none of the seeds bore any sort of pro-embryo at 35 DAP. Embryos at fifty DAP were in globular shape and at later stages the seeds carried one or more developed embryos. The first embryo which developed from globular shape to a normal and mature embryo was a zygotic one, recognition of which from nucellar embryos might have great. impact on breeding of citrus species. In this study it was shown that the genotype and fruit age significantly affect the phenomenon of polyembryony as well as diameter and weight of fruits.
 
Keyword(s): CITRUS, NUCELLAR EMBRYO, GENERETIVE EMBRYO, ROOTSTOCKS, DEVELOPMENTAL STAGE
 
References: 
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