Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   2006 , Volume 42 , Number 2 (166); Page(s) 359 To 374.
 
Paper: 

FUMONISIN B1 CONTAMINATION OF GOLESTAN CORN PRODUCT

 
 
Author(s):  MIRABOU ALFATHI M.*, KARAMI OSBOU R.A., AMINI HASAN
 
* MYCOTOXIN RESEARCH LAB OF PLANT PEST AND DISEASE RESEARCH INSTITUTE, TEHRAN
 
Abstract: 

Amount of fumonisin B1 in 46 corn samples which were collected preharvest, at harvest and post harvest stages (after drying, befor and after silage) from Golestan province in northern Iran was evaluated. The samples were milled using analytical Romer mill. Fumonisin B1 was extracted with methanol-water (80:20 v/v) solvent, purified by immunoaffmity columns (IACs) and converted to fluorescent derivatives by OPA (Ortho-phetaldehyde).The mycotoxin was quantified through high-performance liquid chromatography (HPLC) with fluorescence detector (Em=335 nm, Exi=440 nm). Standard curve was drawn between 0.3125-40 mg/ml using 6 fumonisin working standards. Validation of the method was estimated by Certified Reference Material (CRM). The percentage mean of recovery obtained using this method was 90.7 %. All samples were contaminated with fumonisin B1, the range of contamination varied from 261 to 6891 ng/g and the mean of contamination was 2658.35 ng/g. There was in significant difference on the contamination of samples at different sampling stages.

 
Keyword(s): FUMONISIN B1, GOLESTAN PROVINCE, CORN, HPLC, PRE HARVEST, AT HARVEST, POST HARVEST
 
References: 
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