Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   2006 , Volume 42 , Number 2 (166); Page(s) 337 To 358.
 
Paper: 

DIVERSITY OF AGROBACTERLUM TUMEFACIENS STRAINS IN IRAN

 
 
Author(s):  SALEHI SANAZ*, RAHIMIAN H.A., GHASEMI A.A.GH.
 
* IRANIAN RESEARCH INSTITUTE OF PLANT PROTECTION
 
Abstract: 

Crown gall caused by Agrobacterium spp. has been observed in various crop species and in several provinces of Iran in recent years. In an attempt to identify the Agrobacterium species causing the disease in different areas of the country, strains were isolated from various infected crop species and characterized by phenotypic features, electrophoretic profile of cell proteins and BOX IR fingerprinting.
On the basis of their phenotypic features, the strains were differentiated into three separate groups. The phenotypic characteristics of the first group of strains were closely similar to those of A. tumefaciens (Rhizobium tumefaciens). Members of the second group, isolated from infected grapevines were also identified as A. tumefaciens. Strains of the third group were phenotypically different from all described species, but appeared to be more similar to A. tumefaciens. These strains were not able to grow on any of the selective media tested and displayed a limited host range, infecting mainly sweet cherry All strains had a similar plasmid profile. The electrophoretic profile of cell proteins of the strains was different, although some similarity of the pattern was noted in the high-molecular weight region.

In fingerprinting using BOX IR primer, a high level of heterogeneity was observed, nonetheless two major groups were differentiated.

Phenotypic features appeared to be reliable criteria in identification of Agrobacterium species encountered in this study.

 
Keyword(s): AGROBACTERIUM TUMEFACIENS, PROTEIN, PLASMID PROFILE, BOX-PCR
 
References: 
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