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Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   2006 , Volume 42 , Number 2 (166); Page(s) 223 To 240.
 
Paper: 

IDENTIFICATLON, DISTRIBUTION AND PREVALENCE OF GRAPEVINE LEAFROLL ASSOCIATED VIRUSES AND GRAPEVINE VIRUS A IN IRAN AND THEIR RATE OF INCIDENCE IN GRAPEVINE CULTIVARS

 
 
Author(s):  ROUMI V., AFSHARIFAR A.R.*, IZADPANAH K.A.
 
* DEPT. OF PLANT PROTECTION, COLLEGE OF AGRICULTURE, SHIRAZ UNIVERSITY, SHIRAZ, IRAN
 
Abstract: 

Grapevine leafroll is one of the most important virus diseases of grapevine in all grape growing countries. This disease causes poor pigmentation, delayed maturity and up to 40% yield reduction of grapes. These symptoms are produced by a closterovirus and a few ampeloviruses which are called Grapevine leaf-roll associated viruses (GLRavs). Nine filamentous viruses referred to as GLRaVs 1 to 9 (all belonging to the family Closteroviridae) have been found associated with the disease. Furthermore, grapevine virus A (GYA), a vitivirus, is also associated with leafroll syndrome as well as with rugose wood complex. Although GLRaV-1 and GYA have already been reported from Iran, little information is available on the incidence and prevalence of these viruses as well as the presence of other grapevine leafroll-associated viruses in this country. In this work the presence and prevalence of grapevine leafroll-associated viruses (GLRav-1, -2, -3, -4, -5 and -9) and GYA in vineyards of four Iranian provinces, namely, Fars, Eastern Azarbaijan, Western Azarbaijan and Kohgiluieh-Boyerahmad were investigated. in addition, the rate of incidence of these viruses in vineyards of the four provinces was determined. For this purpose total RNA was extracted from scraped bark tissues of grapevines and used in one-tube RT-PCR with specific primers to analyze the samples the expected products of GVA and GLRaVs -1. -2. -4 and -5 were obtained from diseased samples collected from all provinces. However in regard to the GLRaV-9 which was found only in Fars and Kohgiluieh-Boyerahmad the size of PCR fragments was smaller than expected. No PCR product was found in healthy control plants. The results from analysis of 609 samples revealed the wide distribution of GVA and some of the GLRaVs in vineyards of the four provinces. GVA was the most prevalent one (18.22%) followed by GLRaV-1 (13.46%). Representing 38.7 and 24.5% of the total infected samples, respectively certain samples contained GLRaV-1 +GV A, GLRaV-2+GV A or GLRaV-4, -5, -9 at the same time. Among the Iranian grapevine varieties the lowest infection rate was recorded in Ghezelozom variety in W-Azarbaijan province while Rishbaba variety in fars province had the highest infection rate.

 
Keyword(s): GRAPEVINE ASSOCIATED LEAFROLL VIRUSES (GLRAVS), GRAPEVINE VIRUS A (GV A), GRAPEVINE VIRUSES, IRAN
 
References: 
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  Persian Abstract Yearly Visit 97
 
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