Paper Information

Journal:   IRANIAN JOURNAL OF PLANT PATHOLOGY   2006 , Volume 42 , Number 2 (166); Page(s) 201 To 221.
 
Paper: 

FORMATION OF TELEOMORPH IN ISOLATES OF RHIZOCTONIA SOLANI UNDER LABORATORY CONDITIONS

 
 
Author(s):  HADIZADEH I., BANIHASHEMI Z.A.D.*
 
* DEPARTMENT OF PLANT PROTECTION, COLLEGE OF AGRICULTURE, SHIRAZ UNIVERSITY, SHIRAZ, IRAN
 
Abstract: 

In order to obtain a repeatable, and reliable method for production of teleomorph (the perfect state) and sporulating of Rhizoctonia isolates, five techniques were used: soil- over lay on culture media, transferring from nutrient- rich to nutrient- poor media, inoculation on stem of plant, use of fungistatic material( agar with a fungicide) and fruiting on water surface. In this research, seventeen multinucleate isolates of R. solani belonging to eleven Anastomosis Groups (AG) and nine isolates from different hosts have been used. The perfect stage consisted typically of small botryose clusters of spore- producing basidium and basidiospore were formed in isolates belonging to AG-3, AG-1, AG-4 (from USA) and subgroup AG-1-I-C by use of transferring from a rich nutrient agar (primary growth media) to nutritionally poor agar (sporulation media) method and only under light - dark period (12h light and 12h dark) conditions. Other AGs did not display the basidial stage under this condition. Sporulating basidial clusters were produced and scattered on and within medium, sometimes on the glass sides and Petri dish. Basidiospore began developing approximately 12 to 17 days after transfer and was lasted for 14- 16 days, On the basis of fruiting body (basidium and basidiospore) characteristics,these isolates were confirmed to be Thanatephorus cucumeris. AG-1-I-C isolates were able to produce more amounts of hymenia and basidiospores (basidial clusters) compared to the others. Hymenia were induced on TWA media but no fruiting occurred on SEA. This successful method was repeatable and during 3 trials, basidiospore was observed in all of the four isolates. In other methods, basidium like structures from profuse branching of the hyphae, production of monilial cells and dense growth of fungi were observed but no basidiospore was found.

 
Keyword(s): THANATEPHORUS CUCUMERIS, RHIZOCTONIA SOLANI, ANASTOMOSIS GROUP, BASIDIUM AND BASIDIOSPORE
 
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