Paper Information

Journal:   IRANIAN JOURNAL OF OBSTETRICS, GYNECOLOGY AND INFERTILITY   FALL-WINTER 2008 , Volume 10 , Number 2; Page(s) 127 To 132.
 
Paper: 

ANALYSIS OF SEX CHROMOSOMES DISORDERS IN PREIMPLANTATION GENETIC DIAGNOSIS

 
 
Author(s):  SAHEB KASHAF H.*, ALE YASIN ASHRAF, KEY NEZHAD P., SAHEBKASHAF S., JAHAN SHAD F., MOHAMMADI ZIAZI L.
 
* NATIONAL INSTITUTE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY, TEHRAN, IRAN
 
Abstract: 

Introduction: Preimplantation genetic diagnosis (PGD) is an advanced diagnostic tool in the assisted reproductive technologies (ARTs) to avoid inheritance of genetic disease by transferring unaffected embryos. The presence of numerical sex chromosome abnormalities was studied using fluorescence in situ hybridization (FISH) with two sex chromosome specific probes for X and Y chromosomes.
Material and Methods: In this experimental study, PGD has been performed for 10 couples, who were carriers of inherited X linked disorders. Conventional hormone therapy, intracytoplasmic sperm injection, blastomer, biopsy and fluorescent in situ hybridization were carried out for all candidates.
Results: Data was analyzed by descriptive statistics.In FISH analysis 93% of cells provides clear signals for X and Y chromosomes from that 29 (XX), 18 (XY), 4 (XXY), 1 (XO) were diagnosed. Transferring of embryos into uterus was performed in 10 cycles resulting into two pregnancies and one born baby. The implantation rate of embryos was 20% and the take home baby rate was 10% following ten PGD experiments.
Conclusion: Chromosomal disorders more frequently in embryos with dismorphology especially in the embryos from older women.

 
Keyword(s): PREIMPLANTATION GENETIC DIAGNOSIS, SEX CHROMOSOMES, INVITRO FERTILIZATION, EMBRYO BIOPSY, FISH
 
References: 
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