Paper Information

Journal:   IRANIAN JOURNAL OF CROP SCIENCES   FALL 2004 , Volume 6 , Number 3; Page(s) 0 To 0.
 
Paper: 

EFFECT OF MICROSPORE DENSITY AND EMBRYO SIZE ON EMBRYOGENESIS, ROOTING, SHOOTING AND SECONDARY EMBRYOGENESIS IN RAPESEED (BRASSICA NAPUS L. CV. GLOBAL)

 
 
Author(s):  ABD ELAHI M.R., MOEENI AHMAD, JALALI JAVARAN MOKHTAR, HADADI P.
 
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Abstract: 

The aim of this study was production of haploid plants, using microspore culture method, in to be used in rapeseed breeding programs. Donor plants (cv. Global) were grown at a day/night temperature of 15/10˚C
(16/8 hours). After 90 days microspores were isolated from buds 2.5-3.5 mm containing microspores at late-uninuclate to early-binucluate stages and cultured in a modified medium (NLN-13). Samples were incubated at 30˚C and in darkness for 14 days then transferred to a shaker at 25˚C for 20 days. In this experiment, the effect of different microspore densities included of: 60000, 40000 and 20000 microspores per ml, was used to study the embryogenesis of microspores. Indeed, effect of embryo size (2-3, 3-4, 4-5, 5-6 and greater than 6 mm)
on seedling germination properties like shoot formation, secondary embryogenesis and root formation were also studied. Results showed significant differences between various culture densities (p<0/01). The density of 60000 microspores produced 1031 embryos in each petri dish (containing 12/5 ml culture medium) and was the best density in this study. The size of embryo showed significant effects on shoot and root formation at 1% of probability level. The size of embryo also showed significant differences on secondary embryogenesis at 5%. Probably level in this expriment, it was observed that 73.33 % of embryos greater than 6 mm produced normal shoot had the best form of germination. In this study 43% of embryos were also regenerated.

 
Keyword(s): BRASSICA NAPUS L., RAPESEED, MICROSPORE CULTURE, EMBRYOGENESIS, REGENERATION
 
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