Paper Information

Journal:   JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY   2006 , Volume - , Number 2 (37); Page(s) 17 To 21.
 
Paper: 

GAMMA RADIATION INACTIVATION OF FMD VIRUS TYPE A87/IRN IN ORDER TO PREPARATION OF KILLED VACCINE

 
 
Author(s):  MOTAMEDI SEDEH F.*, KHORASANI A., SHAFAEI S.K.A.D., SALEHIZADEH M., FATH ELAHI H., ARBABI K., MAJD F.
 
* NUCLEAR RESEARCH CENTRE FOR AGRICULTURE AND MEDICINE, AEOI, KARAJ, IRAN
 
Abstract: 

In this research FMD virus type A87/IRN was used. The virus was multiplied on a BHK2I cell line. Then, the virus titration was detected by TCID50% (Tissue Culture Infection Dose 50%) method, and it was 107.5/ml. The FMD virus was irradiated by gamma ray from 60CO source in-4 till 4oC. The gamma cell, model Issledovapel-PX-30, with the dose rate of 0.551 Gy/sec was applied. Different doses of gamma ray were applied and 6 times were repeated for each dose. Antigenicity and infectivity of the irradiated and control virus samples were studied by Complement Fixation, and Cell Culture methods, respectively. The dose/survival curve for the FMD virus was drawn, according to the curve and D10 Value factor (Dose of gamma ray that decrease one logarithmic cycle of virus population) was obtained, and the optimum dose for inactivation of FMDV type A87/IRN and the unalteration its antigenicity of 40-44kGy was obtained.

 
Keyword(s): VACCINES, VIRUSES, INACTIVATION, GAMMA RADIATION, IRRADIATION, LIVESTOCK, FOOT AND MOUTH DISEASE
 
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