Paper Information

Journal:   IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)   2006 , Volume 37-1 , Number 3 (AGRONOMY & CROP BIOTECHNOLOGY); Page(s) 515 To 520.
 
Paper: 

REACTION OF SOME SOYBEAN CULTIVARS TO CHARCOAL ROT

 
 
Author(s):  NARJESI V., ZEYNALI KHANEGHAH H.*, ZALI A.A., BIHAMTA M.R.
 
* UNIVERSITY COLLEGE OF AGRICULTURE & NATURAL RESOURCES, UNIVERSITY OF TEHRAN, KARAJ
 
Abstract: 

Twenty-five soybean cultivars from different maturity groups were evaluated in artificial infection conditions with two isolates of Tiallosporella phaseolina, in a factorial experiment on the basis of RCBD with two replications in greenhouse, to determine their reactions to the above pathogenic fungus. In order to establish infection in soybean cultivars, autoclaved soil (10 min, 121) was contaminated with inoculums propagated on 50 ml corn meal and sand medium in a 250ml flask. The mixture of sand and corn meal was autoclaved for two successive days and then inoculated with 4-5 agar plugs of the 4 day-old fungus grown on PDA (potato-dextrose agar) at 30oC. Ten gr of inoculums was mixed thoroughly with the soil in each pot. Each soybean cultivar was six times planted in six pots, five seeds in each pot. Two pots were contaminated with isolate I and the other two with isolate II, while the last two pots being used as control (without any contamination). Microsclerotial propagules in lower stem and taproot tissues at growth stage R7 were used to determine infection percentage in cultivars. Analysis of variance showed that there were significant differences among cultivars as to the disease. Some cultivars were susceptible, while some being moderately resistant to T.phaseolina. Based on Duncan's multiple mean comparison test, cultivars NE 3297, Gorgan 3, M58, and Calhoan suffered the highest, while cultivars L93-3258, Macon, Probest and Salin the lowest infection percentage. Cluster analysis, performed on cultivars for infection percentage, placed cultivar NE3297 in a separate cluster.

 
Keyword(s): SOYBEAN, CHARCOALROT, MICROSCLEROTIA, INFECTION PERCENT, SUSCEPTIBLE, RESISTANT
 
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