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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   2008 , Volume 37 , Number 1; Page(s) 99 To 102.
 
Paper: 

INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN

 
 
Author(s):  SALARI M.H.*, BADAMI N., SADEGHIFARD NOUR KHODA, AMIN HARATI F.
 
* DEPT. OF PATHOBIOLOGY, SCHOOL OF PUBLIC HEALTH, MEDICAL SCIENCES, UNIVERSITY OF TEHRAN, IRAN
 
Abstract: 

Background: Clostridium difficile is the most common cause of nosocomial diarrhea. It is usually a consequence of antibiotic treatment, but sporadic cases can occur. The purpose of this study was to investigate five tissue culture monolayers sensitivity in detection of C. difficile-toxin.
Methods: A total of 402 stool samples from patients with nosocomial diarrhea hospitalized in three hospitals of Tehran University of Medical Sciences (TUMS) were collected. The samples were cultured on a selective cycloserine cefoxitin fructose agar (CCFA) and incubated in anaerobic conditions, at 37
°C for 4 days. Isolates were characterized to species level by conventional biochemical tests. Bacterial cytotoxicity was assayed on five tissue culture monolayers.
Results: Our findings show that of the total patients, 24 toxigenic C. difficile (6%) were isolated. All 24 C. difficile toxins showed cytotoxic effect at ≥ 1:10 dilution on Hela, Hep2, Vero, McCoy and Mdck cells after 16, 20, 24, 24 and 30 hours, respectively. C. difficile toxin showed cytotoxic effect at ≥ 1:100 dilutions only on Hela cell monolayer after 48 hours.
Conclusion: Hela cell monolayer may be a satisfactory substitute for the detection of C. difficile toxin in clinical specimens.

 
Keyword(s): PATIENTS, DIARRHEA, CLOSTRIDIUM DIFFICILE, TOXIN, CPE, VARIOUS TISSUE CULTURE
 
 
References: 
 
+ Click to Cite.
APA: Copy

SALARI, M., & BADAMI, N., & SADEGHIFARD, N., & AMIN HARATI, F. (2008). INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN. IRANIAN JOURNAL OF PUBLIC HEALTH, 37(1), 99-102. https://www.sid.ir/en/journal/ViewPaper.aspx?id=103811



Vancouver: Copy

SALARI M.H., BADAMI N., SADEGHIFARD NOUR KHODA, AMIN HARATI F.. INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN. IRANIAN JOURNAL OF PUBLIC HEALTH. 2008 [cited 2021May16];37(1):99-102. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=103811



IEEE: Copy

SALARI, M., BADAMI, N., SADEGHIFARD, N., AMIN HARATI, F., 2008. INVESTIGATION OF VARIOUS TISSUE CULTURE MONOLAYERS SENSITIVITY IN DETECTION OF CLOSTRIDIUM DIFFICILE TOXIN. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 37(1), pp.99-102. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=103811.



 
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