Paper Information

Journal:   JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES   OCTOBER-NOVEMBER 2007 , Volume 14 , Number 4; Page(s) 112 To 124.
 
Paper: 

DESIGN AND CONSTRUCTION OF A RECOMBINANT PLASMID PBI121-GLU IN ORDER TO AGROBACTERIUM MEDIATED TRANSFORMATION OF COTTON

 
 
Author(s):  MOHSENPOUR M.*, HABASHI A.A., BABAEIAN JELOUDAR N.A., HABASHI A.A.
 
* DEPT. OF AGRONOMY AND PLANT BREEDING, MAZANDARAN UNIVERSITY, IRAN
 
Abstract: 

Beta 1, 3-glucanase gene derived from barley encodes an antifungal protein and hydrolyzes glucans which are major components of cell walls of many pathogenic fungi. This gene increases plants resistance against fungus infection. In order to transform beta 1, 3-glucanase gene into the cotton, at first a suitable gene cassette was designed by addition of the CaMV35S promoter and the Nos terminator to open reading frame of the glucanase gene in pCaMV vector. Subsequently the complete cassette of glucanase gene was subcloned into T-DNA region of binary vector pBI121 between nptII as a selective marker and Gus as a reporter gene. The pBI121-Glu recombinant plasmid was used for gene transformation via Agrobacterium into the cotton shoot apices. After isolation the cotton shoot apices and inoculation them by Agrobacterium solution, explants were transferred to selective medium containing kanamycin. The surviving and regenerated shoot apices in selective medium that were positive in histochemical assay for Gus gene expression were considered as putative transgenic plants. They should be cared to grow enough for carrying out further molecular analysis to confirm the integration and expression of the interest gene and transformation event.

 
Keyword(s): CLONING, COTTON, GLUCANASE, HISTOCHEMICAL GUS ASSAY
 
References: 
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