Genetic diversity is one of the most important criteria to select parents in breeding programs. The research was carried out in order to determine genetic variability among prennial lucerne based on chemical composition, nutrition values and SDS-PAGE traits. 18 lucernce genotypes were cultivated in a randomized complete block design with two replications. Chemical composision, nutrition values and reserved proteins in seeds and leaves were electrophoresed. Based on cluster analysis of SDS-PAGE, 18 genotypes were grouped into 3 clusters Genotypes 13, 14, 15, 16, 17 and 18 in all SDS-PAGE methods were placed in same group, while genotype 12 was located in separate cluster. Principal components analysis revealed that the crud protein, P and K had the most relative variance amount.