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Paper Information

Journal:   IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)   2006 , Volume 37 , Number 1; Page(s) 23 To 32.
 
Paper: 

INVESTIGATION AND EVALUATION OF LEAF VARIEGATION PATTERNS AND CHLOROPHYLL-DEFICIENT MUTANTS OBTAINED FROM SOMACLONAL VARIATION IN AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL.)

 
 
Author(s):  SHAJIEI K.*, NADERI R., KHALIGHI A.
 
* UNIVERSITY COLLEGE OF AGRICULTURE AND NATURAL RESOURCES, UNIVERSITY OF TEHRAN
 
Abstract: 

In a survey of the commercial greenhouse production of Saintpaulia sp. in Iran, plants with various types of leaf variegation patterns were collected. A recognition of types of variegated patterns in selected plants was accomplished though a comparison of morphological differences in their such variegation patterns, as: 1-The locations in which the chlorophyll-free cells were developed, 2-How the chlorophyll-free cells were outspreaded, distributed and dispersed on each leaf, and 3-The steady state of variegated (chlorophyll-free) tissues which were distributed throughout the plant. An identification guideline for leaf variegation patterns in Saintpaulia adopted from a reliable and authoritative source of reference was used to recognize the various patterns of variegation. In subsequent experiments, leaf tissues segments were prepared from a pink fantasy-flowered semi-miniature Saintpaulia to be cultured on a modified MS basal medium containing BA and IAA hormones for callus proliferation. This was done to induce somaclonal variation to obtain valuable mutants for further study and evaluation. Obtained calluses were subcultured 4-6 times during a six months period after initiation of the experiment, and then transferred to shoot induction medium for shoot regeneration. One thusand seven hundred and fifty new plants were produced from the proliferated calluses, which were then studied and evaluated for detecting the modified morphological characters. In the next stage, the selected cultivars and obtained variants were propagated in perlite medium through cuttings, and under greenhouse conditions to demonstrate their stability in the favored traits. Among plantlets originated from subcultured calluses, various types of leaf chimeras, variegated foliage patterns and some pink fantasy blossoms with various intensities of violet dots were also observed. Investigation and evaluation of somaclonal variation induced variants demonstrated that it is likely to produce various types of leaf chimeras and variegated foliage patterns to improve morphological traits in African violet. Leaf variation characteristics in vegetative progenies that resulted from propagation through cutting of whole chimeral variants were completely eliminated because of single-cell epidermal origin of new shoots, whereas variegation characteristics of variegated variants were stable throughout the one vegetative generation. Mutated traits of flower pigment variants were also stable throughout the one vegetative generation. Altogether, use of tissue culture technique to develop somaclonal variations could result in revealing the genetic potential of African violet to create and improve the traits.

 
Keyword(s): AFRICAN VIOLET (SAINTPAULIA SP.), SOMACLONAL VARIATION, CHLOROPHYLL-FREE CELLS, VARIEGATION, CHIMERAS
 
References: 
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  Persian Abstract Yearly Visit 103
 
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