Paper Information

Journal:   IRANIAN JOURNAL OF IMMUNOLOGY (IJI)   MARCH 2008 , Volume 5 , Number 1; Page(s) 36 To 44.
 
Paper: 

LISTERIA MONOCYTOGENES ACTIVATED DENDRITIC CELL BASED VACCINE FOR PREVENTION OF EXPERIMENTAL TUMOR IN MICE

 
DOI: 

IJIv5i1A3

 
Author(s):  KHAMISABADI MASOUMEH, ARAB S., MOTAMEDI MASOUMEH, KHANSARI NEMAT ELAH, MOAZENI S.M., GHEFLATI ZAHRA, HAJATI JAMSHID*
 
* DEPARTMENT OF IMMUNOLOGY, SCHOOL OF MEDICINE, MEDICAL SCIENCES UNIVERSITY OF TEHRAN
 
Abstract: 

Background: The use of dendritic cells (DCs) as a cellular adjuvant provides a promising approach in immunotherapy of cancer. It has been demonstrated that Listeria monocytogenes activated DCs pulsed ex vivo with tumor antigens trigger a systemic Th1-biased specific immune response and a single dose of this vaccine will cause a consider-able anti tumor immunity.
Objective: The present study was designed to evaluate the ability of multiple doses of tumor antigen-pulsed DCs, matured in the presence of Listeria monocytogenes components in induction of a potent anti-tumor response and the prevention of tumor formation in an experimental model.
Methods: Bone-marrow de-rived DCs (BMDCs) were cultured in the presence of GM-CSF and IL-4. After 5 days, tumor lysates with/without Listeria monocytogenes lysate were added to the culture media for another 2 days. Mice received mature and tumor antigen pulsed dendritic cells subcutaneously in 3 groups. Tumor growth was monitored and two weeks after immunotherapy, cytotoxic activity of CD8+ T cells was evaluated in different groups.
Results: According to the findings, repeated doses of vaccine did not lead to a significant increase in the activity of cytotoxic T cells and decreased tumor growth of immunized animals.
Conclusion: The current study suggests that increased doses of vaccine do not have sufficient efficiency for prevention of tumor induction. Generation of T regulatory responses upon repeated doses of such vaccines should be considered in future investigations

 
Keyword(s): DENDRITIC CELLS, IMMUNOTHERAPY, CANCER, LISTERIA MONOCYTOGENES
 
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