Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES   WINTER 2007 , Volume 3 , Number 1; Page(s) 13 To 18.
 
Paper: 

REPLACEMENT OF SERINE363 AND SERINE375 CODONS BY ALANINE IN RAT MOU-OPIOID RECEPTOR CDNA

 
 
Author(s):  RABANI M.*, SADEGHI H.M.M., OMID SALARI A., LANGARI J., MOAZEN F.
 
* DEPARTMENT OF PHARMACOLOGY AND TOXCICOLOGY, FACULTY OF PHARMACY AND PHARMACEUTICAL SCIENCES, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

The aim of this study was to use site directed mutagenesis technique to construct a vector in which serine363 and serine375 residues of the COOH-terminal portion of the m-opioid receptor (MOR) were substituted by alanine. These constructs are essential in studying G-protein coupled receptor kinase-mediated MOR desensitization. The nested PCR carried out for conversion of serine363 and serine375 to alanine resulted in the production of a band comparable to the expected size of 1400 bp. Restriction analysis of these bands confirmed the integrity of the PCR products. Ligation of the mutated PCR product into pcDNA3 and its digestion with appropriate restriction enzymes further confirmed the integrity of the PCR product and its orientation into the vector.

 
Keyword(s): OPIOID RECEPTOR, PCDNA3, SERINE363, SERINE375
 
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