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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   WINTER 2008 , Volume 9 , Number 4 (36); Page(s) 262 To 267.
 
Paper: 

DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS

 
 
Author(s):  FATHI FARDIN*, JAFARI KERMANI A., MOULA SEYED JAVAD, POULADI A.
 
* ANATOMY DEPARTMENT, CELLULAR AND MOLECULAR RESEARCH CENTER, KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, SANANDAJ, IRAN
 
Abstract: 

Objective: In this investigation Murine Embryonic Stem (ES) cells were differentiated into endothelial cells.
Materials and Methods: Murine ES cells (CCE cell line) exposed to Alpha-MEM medium containing 10% FBS for 4 days. Then obtained Flk-1 (Flk-1: Vascular Endothelial Growth Factor Receptor 2) Positive cells were cultuted in Endothelial Growth Medium-2 (EGM-2) until the last day of experiment. Differentiated cells were evaluated by immunocytochemistry, RT-PCR and Tube Formation Assays.
Results: When the ES cells cultured in collagen coated dishes containing Alpha-MEM & FBS, Flk-1 positive cells were obtained. After transfering Flk-1 positive cells into fibronectin coated dishes containing EGM2, the cells were assumed a relatively uniform endothelial cell morphology and could be propagated and expanded. Immunocytochemical and RT-PCR analysis of differentiated cells showed that they take up acetylated low-density lipoprotein (LDL), express Flk-1, CD31 and bind the BS-I lectin. When placed in Matrigel, these Murine ES cell–derived endothelial cells formed capillary-like structures characteristic of endothelial cells.
Conclusion: ES cell–derived endothelial cells provide a novel means to examine the mechanisms of endothelial cell development, and may open up new therapeutic strategies.

 
Keyword(s): EMBRYONIC STEM CELLS, ENDOTHELIAL CELLS, EGM-2, DIFFERENTIATION
 
 
References: 
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+ Click to Cite.
APA: Copy

FATHI, F., & POULADI, A., & JAFARI KERMANI, A., & MOULA, S. (2008). DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS. CELL JOURNAL (YAKHTEH), 9(4 (36)), 262-267. https://www.sid.ir/en/journal/ViewPaper.aspx?id=103089



Vancouver: Copy

FATHI FARDIN, POULADI A., JAFARI KERMANI A., MOULA SEYED JAVAD. DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS. CELL JOURNAL (YAKHTEH). 2008 [cited 2021May17];9(4 (36)):262-267. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=103089



IEEE: Copy

FATHI, F., POULADI, A., JAFARI KERMANI, A., MOULA, S., 2008. DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS. CELL JOURNAL (YAKHTEH), [online] 9(4 (36)), pp.262-267. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=103089.



 
 
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