Paper Information

Journal:   IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS   FALL 2006 , Volume 22 , Number 3 (33); Page(s) 172 To 179.
 
Paper: 

INVESTIGATION OF IN VITRO CULTURE OF IRANIAN ST JOHNS WORT (HYPERICUM PERFORATUM L.)

 
 
Author(s):  GHAZIAN TAFRISHI G., AZIZI MAJID, FARSI MOHAMMAD
 
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Abstract: 

St Johns Wort (Hypericum perforatum L.) from Hypericaceae is an important medicinal plant, which its secondary metabolites, hypericin and hyperforine, have several medicinal effects such as antidepressant, antiviral, antibacterial and etc. The importance of studying in vitro culture of medicinal plants is optimizing these protocols for subsequent studies about effective factors on biosynthesis of secondary metabolites and applying these methods in improving medicinal plants. Since now there are no report on in vitro culture of Iranian St Johns Wort and for the first time we studied the callogenesis, shoot regeneration and rooting process of this plant. The seeds of Iranian St Johns Wort were collected from Ardebil province and the base growth media was MS and for callogenesis we studied the effect of several levels of 2,4-D (0.25, 0.5, 1,2 and 5 mg/I) and BA (0.25, 0.5, 1 mg/I) or KIN (0.25, 0.5, 1,2 and 5 mg/I) and NAA (0,5, 1,2 and 5 mg/I), Several levels of BA (0.25, 0.5, land 5 mg/I) and several levels of KIN (1,2 and 5 mg/I) accompanied by 1 mg/INAA were used for shoot regeneration in callus. Several levels Of NAA (0, 0.5, 1,2 and 5 mg/I) were used for rooting of the shoots. The growth condition was 25ocand 16/8 hours period for rooting and shoot regeneration, darkness for callogenesis, the results of callogenesis with Duncans Multiple Range Test at 5% showed that highest callus fresh weight (2.1937 gr) was obtained in 0.25 mg/I 2,4-D with 1 mg/I KIN. Results of shoot regeneration in level 5% showed that maximum number of shoots (95 shoots/call and 4 Cm length) obtained in treatment contain 1 mg/INAA with 1mg/l KIN. Results also showed that maximum root number (4,7 roots per shoot and 2.2 Cm length) was in hormone free media.

 
Keyword(s): ST JOHNS WORT, CALLOGENESIS, ROOTING, SHOOTS REGENERATION
 
References: 
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