Paper Information

Journal:   IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH   2006 , Volume 14 , Number 3 (25); Page(s) 155 To 162.
 
Paper: 

IN VITRO CULTURE OF TWO DAMASK ROSE GENOTYPES FROM EAST AND WEST AZARBAYJAN PROVINCES

 
 
Author(s):  GHAMARI ZARE A.*, ASAREH M.H., SHAHRZAD SH., GHORBANLI MAH LAGHA, ALAH VERDI MAMAGHANI B.
 
* RESEARCH INSTITUTE OF FORESTS AND RANGELANDS, TEHRAN, IRAN
 
Abstract: 

Conventional propagation of Rosa damascena is limited attributed to low adventitious root formation on cutting- In vitro culture has become an alternative method for propagation of this plant species. Shoot proliferation and in vitro rooting of two Azarbayjan genotypes using different concentration of growth regulators- West Azarbayjan genotype needed 0.01 mgl-1 TDZ, 0.5 mgl-1 IBA to attain proliferation of only 1.33, while east Azarbayjan genotype required 0.01 mgl-1 TDZ, 0.1 mgl-1 IAA to obtain a maximum shoot multiplication (2.33). Among different concentration of BAP and Kin, the highest multiplication rate (2) in west Azarbayjan genotype was found at 5 mgl-1 BAP. East Azarbayjan genotype reached to its maximum shoot multiplication (3.2) at 2.5 mgl-1 BAP. In order to root regeneration, in vitro propagated shoots were transferred into modified MS medium supplement with different concentration of NAA. East Azarbayjan and west Azarbayjan produced shoots rooted on modified MS medium containing 0.1 and 0.2 mgl-1 NAA, respectively. However, in this research East Azarbayjan genotype had better responses than West Azarbayjan.

 
Keyword(s): ROSA DAMASCENA MILL, PROPAGATION, PLANT GROWTH REGULATORS AND PROLIFRATION
 
References: 
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