Paper Information

Journal:   SHAHID CHAMRAN UNIVERSITY JOURNAL OF SCIENCE   SUMMER 2007 , Volume NEW SERIES , Number 17 (SECTION B); Page(s) 59 To 72.
 
Paper: 

ANTIOXIDANT POTENTIAL OF ASCORBATE AND ALBUMIN IN MERCURY-TREATED BULL SPERMATOZOA

 
Author(s):  ARABI MEHRAN*, SHAHGHOLIAN H.
 
* DEPT. OF BIOLOGY, SHAHREKORD UNIVERSITY, SHAHREKORD, IRAN
 
Abstract: 

Mercury (II) as a harmful metal ion is popularly associated with male reproductive toxicity in animal models and may have the potential to produce adverse effects in men. In the present study, we investigate the antioxidant potential of ascorbate and albumin (BSA), in vitro, in mercuric chloride treated bull spermatozoa. We demonstrated that mercury (100mM) could induce lipid peroxidation (LPO) in spenn samples significantly over three hours. Ascorbate (700 & 1000mM) and BSA (0.25 & 0.5%) depressed the LPO rate, but 1300mM ascorbate and 1% BSA had adverse effect resulting in more LPO. Furthermore, mercury lowered the motility percent and viability of bull sperm cells as well. Here again, ascorbate and. BSA attenuated the negative effects of mercury only in lower concentrations. Mercury produced a reduction in reduced glutathione/GSH levels but co-addition of ascorbate and BSA (low concentrations) with mercury could restore the GSH pool in the bull spermatozoal homogenates. Interestingly, it was found that adding 1300mM ascorbate and 1% BSA may increase GSH levels as a consequence of LPO propagation. These data support that mercury is an active pollutant in nature which may induce infertility in animals by changing the biochemical and physiological aspects of spermatozoa. The researchers may also keep in mind that antioxidants are as double-edged swords when implicated in the narrow concentration ranges.

 
Keyword(s): BULL SPERMATOZOA, ASCORBATE, ALBUMIN, PEROXIDATION, MOTILITY, GSH
 
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