Aflatoxins are highly toxic and carcinogenic mycotoxins produced by Aspergillus spp. Poultry feeds and ingredients are vulnerable to fungal growth and aflatoxin formation. Naturally occurring aflatoxin contains aflatoxins B1, B2, G1 and G2, but aflatoxin B1 is usually the highest concentration and is the most toxic. The most economically significant effects of aflatoxicosis in growing birds are decreasing growth and poor feed conversion rate, decreasing size and egg production and immunosuppressive effects. In the present study 75 samples consist of 25 samples of soyameal, maize and fish meal were collected from 2 main poultry feed factories and poultry farms around Ahvaz. Isolation of fungi was made by pour plate method and aflatoxins extracted by thin layer chromatography (TLC) method. The results of the present study showed that 96 percent of soyameal, 88 percent of maize and 100 percent of fish meal were contaminated with Aspergillus spp with different levels. The highest contaminations of Aspergillus were made from domestic maize and fishmeal. 60% of fishmeal, 40% of maize and 60% of soyameal contained different level of aflatoxin. The highest amount of B1 aflatoxin was 15 ppb detected in domestic fish meal.