Paper Information

Journal:   SHAHID CHAMRAN UNIVERSITY JOURNAL OF SCIENCE   SUMMER 2007 , Volume NEW SERIES , Number 17 (SECTION B); Page(s) 106 To 116.
 
Paper: 

HISTOLOGICAL AND HISTOMETRICAL STUDIES OF THE GUT ASSOCIATED LYMPHOID TISSUE (GAL T) IN THE INTESTINAL BULB AND MAIN INTESTINE OF THE BARBUS SHARPEYI

 
 
Author(s):  NIKBAKHT MORTEZA*, ALBOUGHOBISH N., PEYGHAN RAHIM
 
* DEPT. OF BASIC SCIENCES OF VETERINARY, SHAHID CHAMRAN UNIVERSITY, AHVAZ, IRAN
 
Abstract: 

GALT is a primary lymphoid tissue and a part of mucosal lymphoid system. Numerous reports explaine considerable differences in GALT structure and distribution among various fish species and also different parts of the gut. The histological and immunological recognition of these differences, in addition, is important for diagnosis of aqua culture disease, as well as for vaccination and disease control. In this study, 20 normal, adult, male and female fish with 46.80±1.46 and 48.40±1.57 cm length and 1425±150.97 and 1570±156.53 grams weight were selected respectively, from propagation 0 center of local fishes in Khuzestan province. The water temperature was 29.7oc in the summer. After weighting and biometrical study, the alimentary canal from the beginning of intestinal bulb up to the end of main intestine was separated and tissue samples were taken from anterior, middle and posterior parts of intestinal bulb and main intestine with maximum 0.5 cm thickness. The samples were immersed immediately in Bouin's solution and the sections were made by paraffin embedding method and stained with H & E and PAS staining techniques. The results showed that the lymphoid tissue is present in two different types and in two areas of intestinal bulb and main intestine walls of Barbus Sharpeyi. In the first type, they were scattered in the mucosal epithelium and in the second type, the lymphoid cells were present as an aggregated cord-like structure in the lamina propria or as diffuse type in the submucosa. The majority of lymphoid cells were lymphocyte and lymphoblast with a few macrophages and plasma cells. The most interesting finding was the lack of lymphoid follicles and payers patches in different parts of the Barbus Sharpeyi alimentary canal. Histometrical results showed a significant difference in the density and distribution of intraepithelial lymphocyte in different parts of intestinal bulb and main intestine (P<0.05) but no significant difference was noticed between two sexes (P>0.05). The most intraepithelial cells were located "in the anterior part of intestinal bulb and in the posterior part of main intestine in both sexes. The results suggest that location, shape and density of lymphoid cells of GALT in Barbus Sharpeyi have considerable species differences.

 
Keyword(s): HISTOLOGICAL, HISTOMETRICAL, GALT, INTESTINAL BULB, MAIN INTESTINE, BARBUS SHARPEYI
 
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