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Paper Information

Journal:   IRANIAN JOURNAL OF NUCLEAR MEDICINE   2006 , Volume 14 , Number 26; Page(s) 23 To 27.
 
Paper: 

APPLICATION OF RADIOIMMUNOASSAY TECHNIQUE FOR DETERMINATION OF ANTIGEN CONCENTRATION IN DIFFERENT CELLS WITH A NEW MONOCLONAL ANTIBODY

 
 
Author(s):  ZARABI AHRABI N., MONTASER KOUHSARI SH., BEHRADKIA P., SHAFIEI M., NAJAFI R., BABAEI M.H.*
 
* RADIOISOTOPE DEPARTMENT, NUCLEAR RESEARCH CENTER, AEOI, NORTH KARGAR AVE. TEHRAN, IRAN
 
Abstract: 
Introduction: Binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells, in fact monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kinds of cancers.
Methods: In this research, a new monoclonal antibody against colon cancer cells was prepared and antigen concentration in different cells determined by a radioimmunoassay method using iodine (1-125) labeled protein G.
Results: 125I-labeledprotein G percent binding to white blood cell, HT29, LS 180 and MCF7 cell lines were 7.1%, 91.2%, 75.8% and 40.2%, respectively.
Conclusion: Regarding importance of monoclonal antibody applications, it is necessary to find an efficient method for their evaluation in cancer therapy. In this method, a radioactive agent with no count restriction was used. Also by this method, amount of the antigen can be easily quantified.
 
Keyword(s): MONOCLONAL ANTIBODY, HYBRIDOMA, RADIOIMMUNOASSAY TECHNIQUE, PROTEIN G
 
References: 
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